TaqMan Insights

What is TaqMan?

TaqMan is a PCR assay that uses a fluorogenic probe to enable the quantitative detection of a specific DNA sequence during PCR. The probe contains a reporter dye at the 5' end and a quencher dye at the 3' end. During PCR, if the target sequence is present, the probe will hybridize to the target sequence between the forward and reverse primers. The DNA polymerase will cleave the probe, separating the reporter dye from the quencher dye, resulting in a fluorescent signal that is proportional to the amount of target DNA present in the sample. It is commonly used for gene expression analysis, pathogen detection, and SNP genotyping.

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What other technologies are related to TaqMan?

TaqMan Competitor Technologies

ELISA

ELISA (Enzyme-linked immunosorbent assay) is a plate-based assay technique for detecting and quantifying substances, such as peptides, proteins, antibodies, and hormones. It serves as an alternative method to TaqMan-based qPCR for quantifying target molecules.

mentioned alongside TaqMan in 0% (69) of relevant job posts

TaqMan Complementary Technologies

qPCR

Quantitative PCR (qPCR) is a technique to quantify DNA or RNA. TaqMan is a type of probe commonly used *in* qPCR. Therefore qPCR is complementary, as TaqMan is a component of it.

mentioned alongside TaqMan in 0% (104) of relevant job posts

PCR

PCR is a fundamental technique for amplifying DNA. TaqMan assays are used in conjunction with PCR to detect and quantify the amplified DNA. PCR provides the template for TaqMan assays to work.

mentioned alongside TaqMan in 0% (143) of relevant job posts