Spectral flow cytometry is an advanced flow cytometry technique that measures the entire emission spectrum of fluorescent dyes (fluorophores) used to label cells. Unlike conventional flow cytometry, which measures fluorescence intensity in a few discrete channels, spectral flow cytometry captures a broader range of wavelengths, allowing for the simultaneous detection of more fluorophores and improved resolution of overlapping spectra. This technology is commonly used in immunology, cell biology, and drug discovery to analyze complex cell populations, identify rare cell types, and study cellular functions with high precision.
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