What is RTPCR?

Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique combining reverse transcription of RNA into DNA and amplification of specific DNA targets using polymerase chain reaction (PCR). It is primarily used to measure the amount of a specific RNA, often to quantify gene expression. RT-PCR is widely used in diagnostics to detect RNA viruses, such as SARS-CoV-2, and in research to study gene activity.