Tech Insights

RNA purification

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What is RNA purification?

RNA purification is a process used to isolate RNA from a sample, separating it from other cellular components like DNA, proteins, and lipids. It is a crucial step in many molecular biology techniques, including gene expression analysis (e.g., RT-PCR, RNA sequencing), northern blotting, and the creation of cDNA libraries. Common methods include liquid-liquid extraction (e.g., using phenol-chloroform), silica-based column purification, and magnetic bead-based purification.

What other technologies are related to RNA purification?

RNA purification Complementary Technologies

qPCR (quantitative PCR) is used to quantify RNA levels after RNA purification, making it a strongly complementary technology.
mentioned alongside RNA purification in 0% (57) of relevant job posts
PCR can be used on cDNA generated from purified RNA (via reverse transcription) to amplify specific sequences. Thus, RNA purification is often used as a prep step for PCR.
mentioned alongside RNA purification in 0% (60) of relevant job posts

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