RNA purification is a process used to isolate RNA from a sample, separating it from other cellular components like DNA, proteins, and lipids. It is a crucial step in many molecular biology techniques, including gene expression analysis (e.g., RT-PCR, RNA sequencing), northern blotting, and the creation of cDNA libraries. Common methods include liquid-liquid extraction (e.g., using phenol-chloroform), silica-based column purification, and magnetic bead-based purification.
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