What is qPCR/ddPCR?

qPCR (quantitative PCR) and ddPCR (droplet digital PCR) are both powerful techniques used to amplify and quantify specific DNA or RNA sequences. qPCR measures the amplification in real-time, providing data on the amount of target sequence present. ddPCR partitions the sample into thousands of droplets, amplifies the target in each droplet individually, and then counts the number of positive droplets to achieve absolute quantification. Both are used in gene expression analysis, pathogen detection, mutation detection, and other applications where precise quantification of nucleic acids is crucial.