What is Perturb-seq?

Perturb-seq is a powerful technique that combines CRISPR-based gene editing with single-cell RNA sequencing (scRNA-seq) to simultaneously perturb multiple genes and measure the resulting changes in gene expression at a single-cell resolution. This allows researchers to study the effects of genetic perturbations on cellular phenotypes and biological pathways in a high-throughput manner, and uncover gene regulatory networks. It is commonly used to identify drug targets, understand disease mechanisms, and discover novel gene functions.