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nucleofection

nucleofection

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What is nucleofection?

Nucleofection is a non-viral transfection method used to deliver nucleic acids (DNA, RNA, siRNA, etc.) directly into the cell's nucleus. It utilizes electroporation, applying a brief electrical pulse to create transient pores in both the cell membrane and the nuclear membrane, enabling the direct passage of the nucleic acid payload into the nucleus. It is commonly used for gene editing, protein expression, and cell reprogramming, particularly in cells that are difficult to transfect using other methods.

What other technologies are related to nucleofection?

nucleofection Complementary Technologies

Flow cytometry can be used to analyze the efficiency of nucleofection by quantifying the number of cells that have successfully taken up the introduced material (e.g., DNA, RNA, protein) or to assess the impact of the introduced material on cell phenotype or function.
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