NanoBRET is a bioluminescence resonance energy transfer (BRET) technology used to measure protein-protein interactions in live cells. It utilizes a NanoLuc luciferase donor and a fluorescent protein acceptor. When the donor and acceptor are in close proximity (indicating protein interaction), energy is transferred from the donor to the acceptor, resulting in a shift in light emission. This allows researchers to quantitatively assess protein interactions within a cellular environment.
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