Luciferase assays are bioluminescent assays used to study gene expression, protein-protein interactions, and other biological processes. They rely on the light-emitting reaction catalyzed by luciferase enzymes. Commonly, the luciferase gene is fused to a promoter of interest, and the resulting light output, measured with a luminometer, reflects the activity of that promoter. These assays are widely used in drug discovery, basic research, and diagnostics due to their sensitivity, ease of use, and high-throughput capabilities.
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