Ligation, in molecular biology, generally refers to the process of joining two nucleic acid fragments. It is widely used in recombinant DNA technology to insert DNA fragments into plasmids or other vectors, allowing for the creation of new DNA constructs. DNA ligase is the enzyme that facilitates this process by forming a phosphodiester bond between the 3'-hydroxyl and 5'-phosphate ends of adjacent nucleotides in DNA or RNA.
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