immunoblots

What is immunoblots?

Immunoblotting, also known as Western blotting, is a widely used analytical technique in molecular biology and immunology to detect specific proteins in a sample of tissue homogenate or extract. It involves separating proteins by gel electrophoresis, transferring them to a membrane, and then probing the membrane with antibodies specific to the target protein. The bound antibodies are then detected, allowing for the identification and quantification of the protein of interest. Immunoblots are commonly used to study protein expression, post-translational modifications, and protein-protein interactions.