Illumina sequencers are a widely used technology for massively parallel DNA sequencing. They work by attaching fragmented DNA to a flow cell surface, amplifying these fragments to create clusters, and then sequencing these clusters using sequencing-by-synthesis chemistry. During each cycle, a fluorescently labeled nucleotide is added to the DNA strand, and the sequencer detects the fluorescence to determine the base. Illumina sequencers are commonly used in genomics research, diagnostics, and personalized medicine for applications such as whole-genome sequencing, RNA sequencing, targeted sequencing, and metagenomics.
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