HILIC Insights

What is HILIC?

Hydrophilic Interaction Chromatography (HILIC) is a variant of normal-phase liquid chromatography that separates analytes based on their polarity. HILIC typically uses a polar stationary phase and a mobile phase consisting of a high percentage of organic solvent. It is commonly used to separate polar compounds that are poorly retained in reversed-phase liquid chromatography (RPLC).

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What other technologies are related to HILIC?

HILIC Competitor Technologies

RP

Reversed-Phase (RP) chromatography is a very common alternative separation technique to HILIC, often used for similar types of analytes, making them competitors.

mentioned alongside HILIC in 10% (101) of relevant job posts

HILIC Complementary Technologies

LC-MS

Liquid Chromatography-Mass Spectrometry (LC-MS) is often used with HILIC to provide both separation and identification/quantification of analytes. HILIC provides the separation, and MS provides the detection and identification.

mentioned alongside HILIC in 1% (81) of relevant job posts

mass spectrometry

Mass spectrometry (MS) is a detection technique frequently coupled with chromatographic methods like HILIC. HILIC separates the compounds, and MS identifies and quantifies them.

mentioned alongside HILIC in 1% (62) of relevant job posts