FACS (Fluorescence-Activated Cell Sorting) sorting is a specialized type of flow cytometry used to isolate specific cell populations from a heterogeneous mixture. Cells are labeled with fluorescent antibodies that bind to specific cell surface or intracellular markers. These labeled cells are then passed through a flow cytometer, where they are individually analyzed for their fluorescent properties. Based on these properties, the instrument can physically separate and collect the desired cell populations into different tubes or wells for further analysis or experimentation. It is commonly used in immunology, cancer research, and stem cell biology for isolating specific cell types for downstream applications like cell culture, genomics, and functional assays.
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