FACS analysis Insights

What is FACS analysis?

FACS (Fluorescence-Activated Cell Sorting) analysis, also known as flow cytometry, is a laser-based biophysical technology employed in cell counting, cell sorting, biomarker detection and protein engineering, by suspending cells in a stream of fluid and passing them by an electronic detection apparatus. It allows simultaneous multiparametric analysis of the physical and/or chemical characteristics of single cells flowing through an optical and/or electronic detection system. It is commonly used in immunology, cancer research, and infectious disease studies to identify and quantify different cell populations, assess cell viability, and analyze cell surface and intracellular markers.

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What other technologies are related to FACS analysis?

FACS analysis Complementary Technologies

PCR

PCR (Polymerase Chain Reaction) is a molecular biology technique used to amplify specific DNA sequences. While not directly competitive with FACS, PCR can be used to prepare samples for FACS analysis, for example, to identify specific cell populations based on gene expression after sorting.

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ELISA

ELISA (Enzyme-Linked Immunosorbent Assay) is a plate-based assay technique for detecting and quantifying substances such as peptides, proteins, antibodies, and hormones. ELISA can be complementary to FACS, by validating results or providing orthogonal data related to protein expression or antibody binding.

mentioned alongside FACS analysis in 0% (82) of relevant job posts